Journal: Methods in cell biology

Article Title: Analysis of primary cilia in renal tissue and cells

doi: 10.1016/bs.mcb.2019.04.008

Figure Lengend Snippet: Immortalized and primary renal epithelial cells.

Article Snippet: The balance between cilia assembly and disassembly regulates cilia length ( Mirvis, Stearns, & James Nelson, 2018 ; Spalluto, Wilson, & Hearn, 2013 ). table ft1 table-wrap mode="anchored" t5 caption a7 Cell line Description Source References M-1 Murine cortical collecting duct epithelial cells ATCC Stoos et al. (1991) IMCD-3 Murine inner medullary collecting duct epithelial cells ATCC Rauchman et al. (1993) LLC-PK1 Porcine proximal tubule epithelial cells ATCC Nielsen et al. (1998) MDCK Madin-Darby canine kidney epithelial cells ATCC Gaush et al. (1966) NHK/ADPKD Primary cortical epithelial cells from normal human kidney (NHK) or Autosomal Dominant PKD (ADPKD) KUMC Graham et al. (1977) and Reif et al. (2011) Open in a separate window Immortalized and primary renal epithelial cells. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Company Cilia structure References Acetylated-α-tubulin Sigma-Aldrich Axoneme Ishikawa et al. (2012) , Seixas et al. (2015) , Silva et al. (2018) , and Yu, Sharma, Skowronek, and Erdmann (2016) γ-tubulin Sigma-Aldrich Centrioles Breslow et al. (2013) Pericentrin Covance Centrioles Ishikawa et al. (2012) ARL13B Proteintech Ciliary membrane Seixas et al. (2015) INPP5E Proteintech Ciliary membrane Plotnikova et al. (2015) IFT52 Proteintech Axoneme Silva et al. (2018) IFT81 Proteintech Axoneme Silva et al. (2018) IFT88 Proteintech Axoneme Silva et al. (2018) IFT140 Proteintech Axoneme Silva et al. (2018) BBS2 Proteintech Axoneme Silva et al. (2018) BBS5 Proteintech Axoneme Silva et al. (2018) Open in a separate window Markers of primary cilia.

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